IMMUNOGENITY OF 32.2 kDA HEMAGGLUTININ BM PROTEIN OF Escherichia coli PILI ISOLATED FROM INFERTILE MALES’ SEMEN

Folia Medica Indonesiana Vol. 47 No.2 April – June 2011 : 68-73
Oleh : Sukarjati, Doddy M Soebadi, Aucky Hinting, Sudjarwo, S1 – Biologi (sukarjati@unipasby.ac.id)

Keyword : protein hemagglutinin pill of E. coli BM32.2 kDa, immunogenity, indirect elisa, western blot, dot blot

Male genital tract infection is one cause of infertility. Escherichia coli (E. coli) is a major cause of prostatitis and epididymitis that can degrade the quality of spermatozoa. One factor E. coil can reduce sperm quality due to the ability of E. coli attached to the sperm plasma membrane using pill. Have successfully isolated the protein hemagglutinin pili of E. coil isolates were infertile men with BM cement 32.2 kDa. The purpose of this study was to determine the protein hemagglutinin pili immunogenitas E. BM coil isolates 32.2 kDa cement infertile men. Immunization performed on male rabbits using protein hemagglutinin pill of E. coli BM 32.2 kDa as antigens. Antibody titers formed were measured using indirect methods and the results were read using elisa reader. Antigen and antibody reactivity are formed in the test by the method of dot blot and western blot. The results of this research is through indirect elisa test, the protein hemagglutinin pill of E. BM colt isolates 32.2 kDa cement infertile men can stimulate the formation of antibodies. The results of dot blot test and western blot proved that the antibodies react specifically with antigen. The conclusion of this study is the protein hemagglutinin pili of E. coli BM 32.2 kDa is immunogenic and potentially as a candidate vaccine ingredient in preventing male genital tract infections.

PENALARAN DALAM ESAI MAHASISWA UNIPA SURABAYA

BASTRA || Jurnal Penelitian Pendidikan Bahasa Dan Sastra
Volume 4 || Nomor 1 || Juni 2017 || ISSN: 2356-1629
Penerbit : Program Studi Pendidikan Bahasa dan Sastra Indonesia FKIP Universitas PGRI Adi Buana Surabaya
Penulis 1: Tri Indrayanti
Penulis 2: Ira Eko Retnosari
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Abstrak: Artikel ini bertujuan mendeskripsikan penalaran esai dalam paragraf
mahasiswa Prodi PGSD angkatan 2015 Unipa Surabaya. Pendekatan yang digunakan
pada artikel ini adalah deskriptif kualitatif dengan pengumpulan data menggunakan
metode pustaka dan dokumentasi. Prosedur pengumpulan data dalam penelitian ini
adalah (1) pengumpulan esai, (2) pengodean nama-nama mahasiswa, (3)
pengategorian data mahasiswa dengan penalaran paragraf, (4) pengolahan data, (5)
penghitungan persentase hasil. Penganalisisan data dalam penelitian ini menggunakan
metode deskriptif kualitatif. Artinya, hanya dideskripsikan penalaran paragraf. Adapun
tahap-tahap yang digunakan dalam penganalisisan data adalah (1) pengumpulan data,
(2) pengklasifikasian data, dan (3) penginterpretasian. Pemeriksaan keabsahan
penelitian ini menggunakan teknik ketekunan pengamatan, triangulasi, dan pengecekan
teman sejawat. Hasil analisis artikel ini ditemukanpenggunaan penalaran deduktif dan
induktif. Dalam menggunakan kedua penalaran tersebut, mahasiswa prodi PGSD
angkatan 2015 termasuk baik.
Kata kunci: penalaran paragaf, esai, mahasiswa Prodi PGSD

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